![]() ![]() Simplified Testing and Annotation of Existing Primers Easily search all folders for primers that match a sequence of interest with the redesigned Test with Saved Primers operation.ĭrag and Drop Sequence Export Export annotated sequences in GenBank format by simply dragging documents out of Geneious Prime and onto the desktop or into another program.Įasy Extraction of PCR and Restriction Digest Products Shift click between two compatible primers or restriction enzymes for quick and easy extraction of their product from your sequence. ![]() Powerful Primer Annotation Display 5 extensions (tails) on annotated primers are now displayed inside the sequence view to indicate the length of the extension, the nucleotides it contains and what functional elements are present such as restriction sites, spacers and tags. Rapid Manual Primer Design Select desired binding site in the Sequence View to see real-time display of length and melting point (Tm) then easily add a primer annotation with a convenient new button. Import standard formats from public databases or external codon analysis tools. Geneious Prime 2019 2019FullRelease 6 November 2018 2019 release notes Custom Codon Usage Tables Back translate and optimize codons using your own codon usage tables. Off-target information for the tested document will be added to the new primer annotations. Primer Specificity Testing Be confident your primers will only bind in a single location with automatic identification of any additional, off target, binding sites found when using Test with Saved Primers. Import Metadata on to Sequences and other Documents Seamlessly attach new data from downstream analyses or other applications onto your sequences in Geneious, or update document fields, by importing columns from a CSVTSV format spreadsheet onto documents that are already in Geneious.Īnnotate from Database Improvements Easily identify and annotate the best matched features from a database on your plasmids and other sequences using the new extended database of standard plasmid features provided with Geneious, or use your own sequences as reference.Ĭodon Optimization and Back Translation Improvements Customize the codon optimization parameters for your model organism by defining short sequence motifs to exclude from your optimized sequence, while easily generating multiple co-optimized versions of your sequence simultaneously. ![]()
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